Evolutionary Engineering

古旗祐一 / Yuichi FURUHATA (Ph.D)

古旗 祐一 Yuichi FURUHATA

学歴

2009.03 私立武蔵高等学校 卒業
2013.03 東京大学教養学部生命認知・科学科 卒業
2015.03 東京大学大学院新領域創成科学研究科メディカルゲノム専攻修士課程 修了
2018.03 東京大学大学院新領域創成科学研究科メディカル情報生命専攻博士課程 修了
2018.03 医科学博士取得

職歴

2015.04-2018.03 日本学術振興会特別研究員（DC1）
2018.04-present 産業技術総合研究所研究員
2022.07-present（米国留学中） University of California, Irvine; Specialist

発表論文

  1. Furuhata Y, Sasaki A, "Monitoring Molecular Properties of a Fluorescence Light-Up Aptamer Using Fluorescence Cross-Correlation Spectroscopy", Applied Sciences, 12, 2002 (2022).
  2. Viswan A, Yamagishi A, Hoshi M, Furuhata Y , Kato Y , Makimoto N, Takeshita T, Kobayashi T, Iwata F, Kimura M, Yoshizumi T, Nakamura C, "Microneedle Array-Assisted, Direct Delivery of Genome-Editing Proteins Into Plant Tissue", Frontiers in Plant Science, 13, 878059 (2022).
  3. Furuhata Y, Kato Y, "Asymmetric Roles of Two Histidine Residues in Streptococcus pyogenes Cas9 Catalytic Domains upon Chemical Rescue", Biochemistry, 60, 194-200 (2021).
  4. Furuhata Y, Sakai A, Tomi Murakami, Akira Nagasaki, Kato Y, "Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system", PLOS ONE, 15, e0227477 (2020).
  5. Furuhata Y, Sakai A, Kato Y, "Protein electroporation of Cre recombinase into cultured Arabidopsis cells with an intact cell wall", Protocol Exchange, 27 (2019).
  6. Furuhata Y*, Kobayashi M, Maruyama R, Sato Y, Makino K, Michiue T, Yui H, Nishizawa S, Yoshimoto K*. "Programmable RNA Detection with a Fluorescent RNA Aptamer Using Optimized Three-way Junction Formation", RNA, 25, 590-599 (2019). *Corresponding authors
  7. Furuhata Y, Sakai A, Murakami T, Morikawa M, Nakamura C, Yoshizumi T, Fujikura U, Nishida K, Kato Y, "A method using electroporation for the protein delivery of Cre recombinase into cultured Arabidopsis cells with an intact cell wall", Scientific reports, 9, 2163 (2019).
  8. Furuhata Y, Nihongaki Y, Sato M, Yoshimoto K. "Control of Adipogenic Differentiation in Mesenchymal Stem Cells via Endogenous Gene Activation Using CRISPR-Cas9", ACS Synthetic Biology, 6, 2191-2197 (2017).
  9. Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M. "CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation" Nature Methods, 14, 963-966 (2017).
  10. Furuhata Y, Yoshitomi T, Kikuchi Y, Sakao M, Yoshimoto K. "Osteogenic Lineage Commitment of Adipose-Derived Stem Cells Is Predetermined by Three-Dimensional Cell Accumulation on Micropatterned Surface", ACS Applied Materials & Interfaces, 9, 9339-9347 (2017).
  11. Furuhata Y, Kikuchi Y, Tomita S, Yoshimoto K. "Small spheroids of adipose-derived stem cells with time-dependent enhancement of IL-8 and VEGF-A secretion", Genes to Cells, 21, 1380-1386 (2016).


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研究費（現行）

GrantL（産総研競争的資金）：代表
GrantL（産総研競争的資金）：分担
持田記念医学薬学振興財団 研究助成：代表

所属学会

日本核酸化学会、日本ゲノム編集学会

連絡先

y-furuhata at aist.go.jp
(please exchange "at " to "@")