Evolutionary Engineering




  1. Oikawa S, Yuan S, Kato Y , Akimoto T, "Skeletal muscle-enriched miRNAs are highly unstable in vivo and may be regulated in a Dicer-independent manner", FEBS J, in press, (2023).
  2. Sanada Y, Ikugta Y, Ding C, Dilmulati Y, Kato Y , Nakasa T, Ishikawa M, Mizuno S, Takahashi S, Huang W, Lotz M, Adachi N, Miyaki S, "miR-26a deficiency is associated with bone loss and reduced muscle strength but does not affect severity of cartilage damage in osteoarthritis", Mechanisms of Ageing and Development, 212, 111806, (2023).
  3. Furuhata Y, Egi E, Murakami T, Kato Y , "A method for electroporation of Cre recombinase protein into intact Nicotiana tabacum cells", Plants, 12(8), 1631, (2023).


  1. Furuhata Y, Sasaki A, "Monitoring Molecular Properties of a Fluorescence Light-Up Aptamer Using Fluorescence Cross-Correlation Spectroscopy", Applied Sciences, 12, 2002 (2022).
  2. Viswan A, Yamagishi A, Hoshi M, Furuhata Y , Kato Y , Makimoto N, Takeshita T, Kobayashi T, Iwata F, Kimura M, Yoshizumi T, Nakamura C, "Microneedle Array-Assisted, Direct Delivery of Genome-Editing Proteins Into Plant Tissue", Frontiers in Plant Science, 13, 878059 (2022).


  1. Sano M, Morishita K, Oikawa S, Akimoto T, Sumaru K, Kato Y, "Live-cell imaging of microRNA expression with post-transcriptional feedback control", Molecular Therapy: Nucleic Acids, 26, 547-556 (2021).
  2. Furuhata Y, Kato Y, "Asymmetric Roles of Two Histidine Residues in Streptococcus pyogenes Cas9 Catalytic Domains upon Chemical Rescue", Biochemistry, 60, 194-200 (2021).


  1. Zhao J, Inomata R, Kato Y, Miyagishi M, "Development of aptamer-based inhibitors for CRISPR/Cas system", Nucleic Acids Research, gkaa865 (2020).
  2. Furuhata Y, Sakai A, Tomi Murakami, Akira Nagasaki, Kato Y, "Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system", PLOS ONE, 15, e0227477 (2020).


  1. Furuhata Y, Sakai A, Kato Y, "Protein electroporation of Cre recombinase into cultured Arabidopsis cells with an intact cell wall", Protocol Exchange, 27 (2019).
  2. Yamagishi A, Matsumoto D, Kato Y, Honda Y, Morikawa M, Iwata F, Kobayashi T, Nakamura C, "Direct delivery of Cas9-sgRNA ribonucleoproteins into cells using a nanoneedle array", Applied Science, 9, 965 (2019).
  3. Furuhata Y*, Kobayashi M, Maruyama R, Sato Y, Makino K, Michiue T, Yui H, Nishizawa S, Yoshimoto K*. "Programmable RNA Detection with a Fluorescent RNA Aptamer Using Optimized Three-way Junction Formation", RNA, 25, 590-599 (2019). *Corresponding authors
  4. Furuhata Y, Sakai A, Murakami T, Morikawa M, Nakamura C, Yoshizumi T, Fujikura U, Nishida K, Kato Y, "A method using electroporation for the protein delivery of Cre recombinase into cultured Arabidopsis cells with an intact cell wall", Scientific reports, 9, 2163 (2019).


  1. Ishitobi H, Sanada Y, Kato Y, Ikuta Y, Shibata S, Yamasaki S, Lotz MK, Matsubara K, Miyaki S, Adachi N, "Carnosic acid attenuates cartilage degeneration through induction of heme oxygenase-1 in human articular chondrocytes", European journal of pharmacology, 830, 1-8 (2018).
  2. Nagasaki A, Kato Y, Meguro K, Yamagishi A, Nakamura C, Uyeda TQP, "A genome editing vector that enables easy selection and identification of knockout cells.", Plasmid, 98, 37-44 (2018).
  3. Nishio M, Yamagishi A, Tsukakoshi K, Kato Y, Nakamura C, Ikebukuro K, "Selection and Characterization of DNA Aptamers Against FokI Nuclease Domain", Methods in molecular biology, 1867, 165-174 (2018).

2017 and before