Hiroshi AOKI's Homepage

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Hiroshi AOKI, Dr. Sci.
Research Group Leader
Environmental Function Engineering Research Group
Environmental Management Research Institute
National Institute of Advanced Industrial Science and Technology (AIST)
16-1 Onogawa, Tsukuba, Ibaraki, 305-8569
E-mail: aoki-h(a)aist.go.jp
last updated 2024/12/04
Japanese
Education
  • 2001/03 Dr. Sci., Department of Chemistry, School of Science, The University of Tokyo.
Professional Positions
  • 2024/04–presentProfessor, University of Tsukuba (Collaborative Graduate School Program).
  • 2022/04–2023/03Visiting Lecturer, Rikkyo University.
  • 2021/04–presentResearch Group Leader, Environmental Measurement Research Institute, AIST.
  • 2020/04–presentVisiting Professor, Keio University.
  • 2020/04–presentVisiting Lecturer, Saitama Institute of Technology.
  • 2019/01–presentVisiting Professor, Saitama Institute of Technology.
  • 2016/02–2017/01Associate Specialist, University of California, Irvine.
  • 2012/10–2013/09Planning Officer, Research Planning Office of Environment and Energy, AIST.
  • 2010/04–2012/03Visiting Lecturer, Tokyo Medical and Dental University.
  • 2009/10–2021/03Senior Research Scientist, Environmental Measurement Research Institute, AIST.
  • 2004/07–2009/09Research Scientist, Environmental Measurement Research Institute, AIST.
  • 2003/10–2004/06Postdoctoral Research Scientist, Environmental Measurement Research Institute, AIST.
  • 2001/04–2003/09Japan Society for the Promotion of Science (JSPS) Fellow (PD), The University of Tokyo.

Services for Scientific Communities
- Memberships
  • The Japan Society for Analytical Chemistry
  • The Electrochemical Society of Japan
  • The Polarographic Society of Japan
  • The Sensor IoT Consortium
  • The eDNA Society

    • - Committee/Board
  • Editorial Board Member, Sensors (2024–present)
  • Editorial Board Member, Sensors and Materials (2020–present)
  • Associate Editor, Analytical Sciences (2020–2024)
  • Symposium Organizer, Pacifichem 2021 (2018–2021)
  • Observer Member, Sensor IoT Consortium (2018–present)
  • Board Member, The Japan Society for Analytical Chemistry (Headquarter) (2014–2018)
  • Committee Member, Technical Program Committee, Bio4Apps (2013–2014, 2017–2019)
  • Board Member, The Japan Society for Analytical Chemistry (Kanto Branch) (2012–2020, 2021–present)
  • General Chair, Executive Committee, SISYS 2012 (2012)
    • Present Interests
    • Non-labeling oligonucleotide detection based on hybridization-triggered on/off switching architectures
      Development of novel probe molecules based on hybridization-triggered switching of electrochemical signals and non-labeling DNA or RNA detecting sensors based on the thus developed probes
    • Sensor arrays for detection of oligonucleotide biomarkers aiming at rapid and simple environmental and healthcare diagnoses
      Development of rapid and simple detection techniques with high selectivity for biomarkers for endocrine disrupting chemicals and cancers
    • Searching of novel RNA biomarkers for toxicity testing of environmental chemicals
      Searching of novel RNA biomarkers for stimuli from environmental chemicals with more sensitivity compared to conventional ones.

    ResearchIntro
    Research Achievement

    1. Awards and Fellowships

  • 2024Hot Article Award (Analytical Sciences 2024, 40,501-510)
  • 2023Poster Award (Japan Society for Applied Physics)
  • 2023Poster Award for Young Researchers (Japan Society for Analytical Chemistry)
  • 2022Poster Award for Young Researchers (Japan Society for Analytical Chemistry)
  • 2015Best Poster Award (E&E Forum, AIST)
  • 2015AIST Fellowship (Overseas Research)
  • 2014Best Poster Award (E&E Forum, AIST)
  • 2011Top 10 Excellent Poster Award (13th International Symposium on Electroanalytical Chemistry)
  • 2011Award for Best Poster (LS-BT Joint Workshop, AIST)
  • 2010Highlighted Presentation (Pacifichem 2010)
  • 2009Analytical Chemistry Award for Young Researchers (Japan Society for Analytical Chemistry)
  • 2009Award for Best Poster (Toky Conference 2009)
  • 2007Award for Excellent Poster (1st Joint Workshop on Chem. Sens., Biosens. & Measurement Tech.)
  • 2007Selected Paper for Royal Society of Chemistry Virtual Journal (Analyst 2007, 132, 784-791)
  • 2005Selected Paper for Royal Society of Chemistry Virtual Journal (Analyst 2005, 130, 1478-1482)
  • 2003Selected Paper for Royal Society of Chemistry Virtual Journal (Analyst 2003, 128, 681-685)
  • 2001–2003Japan Society of Promotion of Science (JSPS) Research Fellowship (PD)

    • 2. Papers
    - Original Papers and others

    1. H. Aoki*, M. Kawaguchi, Y. Kumakura, H. Kamo, K. Miura, Y. Hiruta, S. Simizu, D. Citterio* “Sensor Arrays for Electrochemical Detection of PCR-Amplified Genes Extracted from Cells Suspended in Environmental Waters”, Sensors 2024, 24, 7182.
    2. Y. Sato, T. Hamai,* Y. Masaki, H. Aoki, H. Habe* “Comparison of Operating Conditions and Microbial Compositions in an Ethanol-fed Sulfate-reducing Bioreactor for the Treatment of Acid Mine Drainage”, Mine Water and the Environment 2024, accepted.
    3. H. Aoki* “Enhancement of microRNA Detection Signal Based on Au-nanoparticle-catalyzed Ag Accumulation Observed by Electrochemical and Surface Plasmon Resonance Analyses”, Sensors & Materials 2024, 36, 3701-3712.
    4. Y. Sato, K. Hasemi, K. Machikawa, H. Kinjo, N. Yashiro, Y. Iimura, H. Aoki, H. Habe* “Assessing microbial stability and predicting biogas production in full-scale thermophilic dry methane fermentation of municipal solid waste”, Bioresource Technology 2024, 402, 130766.
    5. M. Kawaguchi, H. Aoki*, H. Kamo, K. Miura, Y. Hiruta, S. Simizu, D. Citterio* “Simplified Capture, Extraction and Amplification of Cellular DNA from Water Samples”, Analytical Sciences 2023, 40, 501-510.
    6. H. Aoki*, R. Miyazaki, M. Ohama, M. Murata, K. Asai, G. Ogata, Y. Einaga “Urine Protein Quantification in Human Urine on Boron-Doped Diamond Electrodes based on Electrochemical Reaction of Coomassie Brilliant Blue”, Analyst 2023, 148, 4396-4405.
    7. H. Aoki* "Rapd and Simple Quantification of Urine Protein in Urine", The 83th Analytical Chemistry Workshop, Prospects and Topics 2023, 15.
    8. H. Izumi*, H. Aoki, L. A. Nafie, R. K. Dukor "Effect of Conformational Variability on Seasonable Thermal Stability and Cell Entry of Omicron Variants", ACS Omega 2023, 8, 7111-7118.
    9. H. Aoki* "Material-specific determination based on microscopic observation of single microplastic particles stained with fluorescent dyes", Sensors 2022, 22, 3390.
    10. H. Aoki* "Simple Microplastic Analysis Based on Adsorption of Fluorescent Dyes", The 70th Analytical Chemistry Conference, Prospects and Topics 2022, 7.
    11. H. Aoki* "Sensors with Highly Ordered Nucleotides", Anal. Sci. 2021, 37, 1191-1192.
    12. H. Aoki* "Label-free Ratiometric Electrochemical DNA Sensing Based on β-Cyclodextrin-modified Probe Immobilized on Ferrocene Monolayers", Sensors & Materials 2021, 33, 2857-2865.
    13. H. Aoki*, M. Torimura, H. Habe "Spectroscopic investigation of increased fluorescent intensity of fluorescent dyes when adsorbed onto polystyrene microparticles", Analytical Sciences 2021, 37, 773-779.
    14. Y. Kanekiyo*, Y. Mitani, M. Suda, H. Aoki, H. Minai "Development of a formaldehyde gas sensor that exhibit distinct color change", Sensors and Materials 2020, 1101-1109.
    15. H. Aoki*, H. Tani, K. Nakamura, H. Sato, M. Torimura, T. Nakazato "MicroRNA biomarkers for chemical hazard screening identified by RNA deep sequencing analysis in mouse embryonic stem cells", Toxicology and Applied Pharmacology 2020, 392, 114929.
    16. H. Aoki*, T. Nakazato "Switching on/off of the electroactivity of the hemin/guanine-quadruplex complex as a DNA aptazyme triggered by the response to histamine", Sensors & Materials 2020, 32, 1111-1121.
    17. H. Aoki*, T. Sukegawa, M. Torimura, T. Nakazato "Non-labeling and non-external indicator DNA sensing based on ferrocene-terminated probes immobilized on gold film electrode arrays treated with plasma and acid exposures", Sensors & Materials 2020, 32, 1079-1090.
    18. H. Aoki*, R. M. Corn, B. Matthews "MicroRNA detection on microsensor arrays by SPR imaging measurements with enzymatic signal enhancement", Biosensors & Bioelectronics 2019, 142, 111565.
    19. H. Aoki*, M. Torimura, T. Nakazato "384-Channel electrochemical sensor array chips based on hybridization-triggered switching for simultaneous oligonucleotide detection", Biosensors & Bioelectronics 2019, 136, 76-83.
    20. H. Tani*, T. Matsutani, H. Aoki, K. Nakamura, Y. Hamaguchi, T. Nakazato, M. Hamada "Identification of RNA biomarkers for chemical safety screening in neural cells derived from mouse embryonic stem cells using RNA deep sequencing analysis", Biochemical and Biophysical Research Communications 2019, 512, 641-646.
    21. H. Aoki* "New development of environmental and bio-sensing based on biological responses" (Japanese), Kagakukogyo 2017, 68, 52-59.
    22. H. Aoki* "Development of integrated chip devices for gene detection sensors and their applications to environmental diagnoses" (Japanese), JIR News 2017, 3, 14-17.
    23. H. Tani*, J. Takeshita, H. Aoki, K. Nakamura, R. Abe, A. Toyoda, Y. Endo, S. Miyamoto, M. Gamo, M. Torimura, H. Sato "Effect of methyl p-hydroxybenzoate on the culture of mammalian cell", Drug Discoveries & Therapeutics 2017, 11, 276-280.
    24. K. Takeshima, K. Mizuno, H. Nakahashi, H. Aoki, Y. Kanekiyo* "Ratiometric sensing of hydrogen peroxide utilizing conformational change in fluorescent boronic acid polymers", J. Anal. Method. Chem. 2017, doi.org/10.1155/2017/7829438.
    25. H. Tani*, J. Takeshita, H. Aoki, K. Nakamura, R. Abe, A. Toyoda, Y. Endo, S. Miyamoto, M. Gamo, H. Sato, M. Torimura "Identification of RNA biomarkers for chemical safety screening in mouse embryonic stem cells using RNA deep sequencing analysis", PLos One 2017, 12, e0182032.
    26. H. Wu, X.-L. Wu, Z.-M. Wang*, H. Aoki, S. Kutsuna, K. Jimura, and S. Hayashi "Anchoring titanium dioxide on carbon spheres for high-performance visible light photocatalysis", Appl. Catal., B 2017, 207, 255-266.
    27. H. Aoki* "Simple and rapid environmental measurement and healthcare management based on oligonucleotide analysis", The 77th Analytical Chemistry Workshop, Prospects and Topics 2017, 19.
    28. H. Tani*, J. Takeshita, H. Aoki, R. Abe, A. Toyoda, Y. Endo, S. Miyamoto, M. Gamo, and M. Torimura "Genome-wide gene expression analysis of mouse embryonic stem cells exposed to p-dichlorobenzene", J. Biosci. Bioeng. 2016, 122, 329-333.
    29. H. Aoki* "Blood diagnosis based on 'pop-up' cards" (Japanese),, Bunseki 2016, 11, 496-496
    30. H. Aoki* "Electrochemical label-free nucleotide sensors", Chem. Asian J. 2015, 10, 2560-2573.S
    31. T. Goda, Y. Maeda, R. Matsumoto, M. Torimura, H. Aoki*, Y. Miyahara* "Label-free potentiometry for detecting DNA hybridization using peptide nucleic acid and DNA probes: a dominant effect of electrostatic interaction with underlying surface", Sensors 2013, 13, 267-278.
    32. S. Kanata, T. Nishino*, H. Aoki "Effect of aqueous environment on monolayer of tetrairon(I) single molecule magnet", J. Electrochem. Soc. 2012, 159, H72-H76.
    33. H. Aoki* "Development of novel gene detection methods and their applications to rapid environmental diagnosis" (Japanese), Bunsekikagaku 2012, 61, 763-776.
    34. H. Aoki* "Micromachines desirably to catch, transport, and release proteins" (Japanese), Bunseki 2012, 7, 404-404.
    35. H. Aoki*, A. Kitajima, H. Tao "Electrochemical Sensor Array Chips for Multiple Gene Detection", Sensors & Materials 2010, 22, 327-336.
    36. H. Aoki*, H. Tao "Label-free and "signal-on" DNA detection using a probe DNA terminated with ferrocene and β-cyclodextrin", Supramol. Chem. 2010, 22, 455-460.
    37. H. Aoki*, A. Kitajima, H. Tao "Electrochemical gene sensor arrays prepared using non-contact nanoliter array spotting of gene probes", Anal. Sci. 2010, 26, 367-370.
    38. H. Aoki* "Bacteria and Virus for Influenza" (Japanese), Bunseki 2010, 1, 44-44.
    39. H. Aoki*, H. Tao "Signal enhancement for gene detection based on redox reaction of [Fe(CN)6]4- mediated by ferrocene at the terminal of the peptide nucleic acid as a probe with hybridization-amenable conformational flexibility", Anal. Sci. 2008, 24, 929-934.
    40. H. Aoki*, H. Sato, A. Nemoto, T. Ikeda, H. Tao "Application of high-density sample spotting to laser desorption/ionization mass spectrometry", J. Mass Spectrom. Soc. J. 2008, 56, 223-228.
    41. H. Aoki*, T. Ikeda, M. Torimura, H. Tao "Variable pitch dispensing workstation and its application to the preparation of microsensor arrays", Anal. Sci. 2008, 24, 817-821.
    42. H. Aoki*, H. Tao "Label- and marker-free gene detection based on hybridization-induced conformational flexibility changes in a ferrocene-PNA conjugate probe", Analyst 2007, 132, 784-791.
    43. H. Aoki*, Y. Umezawa, A. Vertova, S. Rondinini "Ion-channel sensors based on ETH 1001 ionophore embedded in charged-alkanethiol self-assembled monolayers on gold electrode surfaces", Anal. Sci. 2006, 22, 1581-1584.
    44. H. Aoki*, H. Tao "DNA detection using PNA-ferrocene conjugates based on their conformational change", 11th International Meeting on Chemical Sensors 2006, T01.1.2#237-.
    45. H. Aoki* "SNPs detection based on 'wire-like' probe DNA" (Japanese), Bunseki 2006, 3, 135-135.
    46. H. Aoki*, H. Tao "Gene sensors based on peptide nucleic acid (PNA) probes: Relationship between sensor sensitivity and probe/target duplex stability", Analyst 2005, 130, 1478-1482.
    47. H. Radecka, I. Szymańska, M. Pietraszkiewicz, O. Pietraszkiewicz, H. Aoki, Y. Umezawa* "Intramolecular ion-channel sensors using gold electrodes immobilized with macrocyclic polyamines for voltammetric detection of adenine nucleotides", Chem. Anal. 2005, 50, 85-102.
    48. H. Aoki*, Y. Umezawa, H. Tao "Development of ion-channel sensors for detection of oligonucleotides", Chem. Sens. 2004, 20 (Suppl. B), 528-529.
    49. Y. Umezawa*, H. Aoki "Ion-channel sensors based on artificial receptors", Anal. Chem. 2004, 76, 320A-326A.
    50. H. Aoki, Y. Umezawa* "Trace analysis of an oligonucleotide with a specific sequence using PNA-based ion-channel sensors", Analyst 2003, 128, 681-685.
    51. H. Aoki, K. Hasegawa, K. Tohda, Y. Umezawa* "Voltammetric detection of inorganic phosphate using ion-channel sensors with self-assembled monolayers of a hydrogen bond-forming receptor", Biosens. Bioelectron. 2003, 18, 261-267.
    52. H. Aoki, Y. Umezawa* "High sensitive ion-channel sensors for detection of oligonucleotides using PNA modified gold electrodes", Electroanalysis 2002, 14, 1405-1410.
    53. H. Aoki, Y. Umezawa* "Ion-channel sensors for electrochemical detection of DNA based on self-assembled PNA monolayers", Nucleic Acids Res. Supplement 2002, 2, 131-132.
    54. Y. Umezawa*, P. Bühlmann, K. Umezawa, N. Hamada, H. Aoki, J. Nakanishi, M. Sato, K. P. Xiao, Y. Nishimura "Potentiometric selectivity coefficients of ion-selective electrodes. Part II. Inorganic anions", Pure Appl. Chem. 2002, 74, 923-994.
    55. H. Aoki, Y. Umezawa*, S. Rondinini, A. Vertova "New voltammetric sensors for alkaline cations based on ionophores incorporated into a self-assembled thiol monolayer", Electrochemical Society Proceedings Series 2001, 2001-18, 4-9.
    56. H. Aoki, P. Bühlmann, Y. Umezawa* "Electrochemical detection of a one-base mismatch in an oligonucleotide using ion-channel sensors with self-assembled PNA monolayers", Electroanalysis 2000, 12, 1272-1276.
    57. V. P. Y. Gadzekpo, P. Bühlmann, K. P. Xiao, H. Aoki, Y. Umezawa* "Development of an ion-channel sensor for heparin detection", Anal. Chim. Acta 2000, 411, 163-173.
    58. R. V. Chamberlain II, K. Slowinska, M. Majda, P. Bühlmann, H. Aoki, Y. Umezawa* "Electrostatically-induced inclusion of anions in cyclodextrin monolayers on electrodes", Langmuir 2000, 16, 1388-1396.
    59. V. P. Y. Gadzekpo, P. Bühlmann, K. Xiao, H. Aoki, Y. Umezawa* "Development of an ion-channel sensor for heparin detection", The 61st Analytical Chemistry Workshop, Prospects and Topics 2000, 17.
    60. V. P. Y. Gadzekpo, K. P. Xiao, H. Aoki, P. Bühlmann, Y. Umezawa* "Voltammetric detection of the polycation protamine by the use of electrodes modified with self-assembled monolayers of thioctic acid", Anal. Chem. 1999, 71, 5109-5115.
    61. H. Aoki, P. Bühlmann, Y. Umezawa* "Self-assembly of a tricarboxylate receptor through thioamide groups and its use for electrochemical detection of protonated amines", J. Electroanal. Chem. 1999, 473, 105-112.
    62. P. Bühlmann, H. Aoki, K. P. Xiao, S. Amemiya, K. Tohda, Y. Umezawa* "Chemical sensing with chemically modified electrodes that mimic gating at biomembranes incorporating ion-channel receptors", Electroanalysis 1998, 10, 1149-1158.
    - Books and others
    1. "Environmental Analysis and Material Dynamics" Bioanalysis, H. Aoki*, T. Nakazato, Panoramic View Reports, Environmental and Energy Field (2019), Japan Science and Technology Agency (JST), Tokyo, Japan, (2019) 415-435.
    2. "Gene", H. Aoki*, In Rapid, Simple, and High-Spec Biosensors (Japanese), Gijutsu Joho Kyokai Inc., Tokyo, Japan (2014) 83-87.
    3. "DNA Sensors", H. Aoki*, in Advanced Analytical Methods, NTS Inc., Tokyo, Japan (2004) 241-246.
    4. "Electrochemical Analysis", H. Aoki*, in Experiments in Analytical Chemistry, Tokyo Kagaku Dojin, Tokyo, Japan (1999) 141-164.

    3. Patents
    - US and Canada patents

    1. H. Aoki*, M. Torimura, H. Tao, T. Ikeda "Variable pitch array spotter", US patent number 9,120,071, September 1, 2015.
    2. H. Aoki*, M. Torimura, H. Tao, T. Ikeda "A dispensing apparatus and a dispensing method", US patent number 8,808,625, August 19, 2014.
    3. H. Aoki*, M. Torimura, H. Tao, T. Ikeda "Variable pitch array spotter", US patent number 8,663,577, March 4, 2014.
    4. H. Aoki*, H. Tao, A. Kitajima "Electrochemical molecular detection probe", US patent number 8,454,820, June 4, 2013.
    5. Y. Umezawa, H. Aoki* "Method for electrochemical detection of nucleobase pair complementarity", CA patent number 2, 441,180, August 12, 2008.
    6. Y. Umezawa, H. Aoki* "Electrochemical detection method of complementarity to nucleic acid bases", US patent number 7,169,614, January 30, 2007.
    - JP patents
    1. H. Aoki*, M. Torimura, H. Tao "Dispensing machine", JP patent number 5,552,623, June 6, 2014.
    2. H. Aoki*, M. Torimura, H. Tao "Method and apparatus for volume determination of small droplets", JP patent number 5,483,134, February 28, 2014.
    3. H. Aoki*, M. Torimura, H. Tao, T. Ikeda "Variable pitch array spotter", JP patent number 5,339,257, August 16, 2013.
    4. H. Aoki*, H. Tao, A. Kitajima "Electrochemical molecular recognition probes and molecular recognition sensors, and electrochemical detection methods based on them", JP patent number 5,334,136, August 9, 2013.
    5. H. Aoki*, H. Tao, M. Torimura "A Method and apparatus for measuring volumes of small droplets", JP patent number 5,283,113, June 7, 2013.
    6. H. Aoki*, M. Torimura, H. Tao, T. Ikeda "Variable Pitch Array Spotter", JP patent number 4,686,769, February 25, 2011.
    7. Y. Umezawa, H. Aoki* "Electrochemical detection method of complementarity to nucleic acid bases", JP patent number 3,956,214, May 5, 2007.

    4. Press Releases

    1. "Development of oligonucleotide detection sensors -rapid and simple methods for diagnosis of biological effect of chemicals in environmental and medical fields-", In Nikkan Kogyo Shinbun, September 5, 2013.
    2. "Japan's AIST looks to commercialize spotter for array printing, MALDI-TOF applications", In GenomeWeb LLC, December 1, 2008.
    3. "AIST developed an array spotter enabling to dispense small liquid samples with high precision", In Handotai Sangyo Shinbun, November 12, 2008.
    4. "Eight-fold automatic dispensing. Rapid dispensing of liquid samples, AIST", In Ibaraki Shinbun, October 6, 2008.
    5. "High-speed spotting for liquid samples, AIST. Sample introduction apparatus", In Nikkei Sangyo Shinbun, October 16, 2008.
    6. "Heparin detection with a ten-fold sensitivity", In Nikkei Sangyo Shinbun, August 8, 2000.

    5. Invited Talks (International)

    1. H. Aoki “Biomedical and Environmental Analysis Based on DNA Sensor Array for Nucleotide Biomarkers”, 10th South Asia Collaborative Symposium on Energy Materials (SACSEM 10th), October 2024, Osaka, Japan.
    2. H. Aoki “Monitoring of Water Ecosystem and Bioremediation for Heavy Metal Decontamination”, 4th AIST-DLSU Joint Workshop, July 2024, Osaka, Japan.
    3. H. Aoki “Novel Environmental Evaluation Techniques Based on Biomolecules as Environmental Indicators”, 24th PhotoLIFE Workshop (3rd AIST-DLSU Joint Symposium), March 2024, Osaka, Japan.
    4. H. Aoki "Molecular recognition triggered electrochemical 'nano-structured switches'", International Chemical Congress of Pacific Basin Societies (Pacifichem) 2021, December 2021, Honolulu, HI, USA.
    5. H. Aoki "Sensor Arrays for Label-Free Detection of Nucleotide Biomarkers towards Healthcare and Environment Management", The International Conference on Flexible and Printed Electronics (ICFPE) 2021, September 2021, Niigata, Japan.
    6. H. Aoki "Environmental and biomedical applications of label-free oligonucleotide sensing arrays based on hybridization-triggered "on/off" switching", The 3rd National Conference on Water Treatment and Reuse of China, March 2019, GuangZhu, China.
    7. H. Aoki "Search of RNA biomarkers for chemical safety screening aiming at 'on-site' oligonucleotide sensor devices feasible in environmental and biomedical fields", Bio4Apps 2017, December 2017, Tokyo, Japan.
    8. H. Aoki "Integrated sensor array chips for simple and rapid diagnosis of biologically-significant RNA sequences", Global Research Efforts on Energy and Nanomaterials (GREEN) 2016, December 2016, Taipei, Taiwan, China.
    9. H. Aoki "Label-free sensor array chips: application to simultaneous oligonucleotide detection", Special Seminar in Kansas State University, October 2016, Manhattan, Kansas, USA.
    10. H. Aoki "Electrochemical label-free nucleotide sensors", Special Seminar in Shanghai Second Polytechnic University, November 2014, Shanghai, China.
    11. H. Aoki "Electrochemical detection of gene expression and related researches in AIST", Science and Technology Lecture in Shanghai Second Polytechnic University, November 2014, Shanghai, China.
    12. H. Aoki "Sensor array chips for label-free gene detection based on ferrocene-terminated probes", Bio4Apps 2014, November 2014, Shanghai, China.
    13. H. Aoki "Electrochemical label-free nucleotide sensors for RNA analysis", Bio4Apps 2013, October 2013, Tokyo, Japan.
    14. H. Aoki, T. Fujikake, M. Torimura "Simultaneous label-free gene detection on integrated gene sensor array chips", International Workshop on Soft Interface Sciences for Young Scientists 2012 (SISYS) 2012, November 2012, Tsukuba, Japan.
    15. H. Aoki "Label-free and reagent-free DNA detection for feasible and rapid diagnosis", Summer Seminar for Bioelectroanalysis in Polish Academy of Sciences, August 2012, Olsztyn, Poland.
    16. H. Aoki, A. Kitajima, H. Tao "Label-free and reagent-free DNA detection based on supramolecular electrochemistry" NANOJASP 2010, November 2010, Barcelona, Spain.
    17. H. Aoki, H. Tao "Gene sensors based on peptide nucleic acid (PNA) probes: Sensor responses and association constants between the probes and targets" Biosensors & Biomaterials Workshop 2005, March 2005, Tsukuba, Japan.
    18. H. Aoki, Y. Umezawa, H. Tao "Development of new electrochemical sensors for toxicogenomics" Toxicogenomics International Forum 2004, October 2004, Kyoto, Japan.