Intelligence Officer,  Ministry of Home Affairs, North Bock, New Delhi, India: 1995

Junior Research Fellow, UGC-CSIR, RRL, Trivandrum, India: 1995-1997

Senior Research Fellow, UGC-CSIR, RRL, Trivandrum, India, 1998-2000

Postdoctoral Fellow, Joint Research Center for Atom Technology, Tsukuba, Japan: 2000-2002

JSPS Postdoctoral Fellow, AIST, Tsukuba, Japan: 2002-2003

Postdoctoral Fellow, Pacific Northwest National Lab., Richland, USA: 2003-2004

Visiting Scientist, University of Texas, Austin, USA: 2007

Visiting Professor, CABB, Kerala University, India: 2007~2012

Research Scientist, AIST, Takamatsu, Japan 8/2004-9/2009

Senior Scientist, AIST, Takamatsu, Japan 10/2009~

Adjunct Professor, Department of Environmental Toxicology, Southern University, USA, 2012-2013

PRESTO  (Sakigake) Researcher, Japan Science and Technology Agency 10/2010~3/2014

Guest Professor, Department of Optical Science and Technology, The University of Tokushima, 4/2012~

Guest Professor, Kwansei Gakuin University, 4/2013~

Visiting Scholar, Department of Chemistry, Osaka University, 4/2013~


Editorial Work

Editor, Nano Reviews

Associate Editor, J. Photochem. Photobiol.C: Photochem. Rev.

Associate Editor, Adv. Phys. Chem.

Editorial Board Member, Open Colloid Sci. J.

Editorial Board Member, J. Bioengg. Biomed. Sci.


Academic Societies

Councilor, Japanese Photochemistry Association (JPA)

Director Board Member, Asian Nanoscience and Nanotechnology Association (ANNA)

Fellow, Royal Society of Chemistry

Member, American Chemical Society

Member, Japanese Photochemistry Association

Member, Asian Photochemistry Association

Member, Chemical Society of Japan


As Current/Former Reviewer

Nature Nanotechnology (NPG); Nature Methods (NPG)

ACS Nano (ACS); The Journal of Physical Chemistry (ACS); Nano Letters (ACS)

Journal of the American Chemical Society (ACS)

Langmuir (ACS); Analytical Chemistry (ACS); Environmental Science and Technology (ACS)

Crystal Growth and Design (ACS); ACS Applied Materials and Interfaces (ACS)

Journal of Photochemistry and Photobiology A: Chemistry (Elsevier)

Journal of Photochemistry and Photobiology C: Photochemistry Reviews (Elsevier)

Molecular and Cellular Probes (Elsevier); Chemical Physics Letters (Elsevier)

Materials Research Bulletin (Elsevier); Spectrochemica Acta (Elsevier)

Expert Opinion in Medical Diagnosis (Informa Pharmascience)

Current Opinion in Molecular Therapeutics (Thomson Reuters)

Journal of Chemical Physics (AIP); Applied Physics Letters (AIP)

Macromolecular Chemistry and Physics (Wiley-VCH)

Advanced Functional Materials (Wiley-VCH); Chem. Eur. J. (Wiley-VCH), etc.


Project Reviewer: Singapore National Research Council (2010~)

Project Reviewer: The Marsden Fund Council, New Zealand (2010~)

Faculty Reviewer: Academia Sinica, Taipei, Taiwan (2010)


Fellow, Royal Society of Chemistry (FRSC), 2011~

Japanese Photochemistry Association Award for Young Scientist, Japan, 2011

Lectureship Price for Young Scientists by the Chemical Society of Japan, 2011

PRESTO Project, Japan Science and Technology Agency (JST), 2010

Distinguished Lectureship Award by the Chemical Society of Japan, 2010.03

Asian and Oceanian Photochemistry Award for Young Scientists, 2010

Most Cited Certificate from Analytical and Bioanalytical Chemistry (Springer), 2010

Invited Scientist, Department of Chemistry and Biochemistry, University of Texas, Austin, 2007

14th Gen-nai prize, Ozaki Foundation, Japan: 2007

PNNL Postdoctoral Fellowship, Pacific Northwest National Laboratory, USA: 2003-2004

JSPS Postdoctoral Fellowship, Nanotechnology Research Institute, AIST, Japan: 2002-2003.

JRCAT Postdoctoral Fellowship, Natl. Instt. Interdiscipl. Res., Japan: 2000-2002

UGC-CSIR Junior and Senior Research Fellowships (NET), Government of India:1995, 96, 97, 98, 99.

Thomas Foundation Top Merit Award, Children’s Friend High School, India: 1987, 88

State Merit Award, Government of Kerala, India: 1985, 1988, 1990.



2217-14 Hayashi-Cho

Takamatsu, Kagawa 761-0395


Phone: (087) 869-3558

Fax: (087) 869-4113

Email: v.biju @

Guest Professor
The University of  Tokushima &
Kwansei Gakuin University

Associate Editor,  J. Photochem.
Photobiol. C: Photochem. Rev.  

Please come back for more images


More pictures coming soon


Propagating single-molecule EGF-EGFR complexes on human ovarian epidermoid carcinoma cells: Amplification of cell signaling


Cytochrome-rich membrane domains of a bacterium


3D Cluster


2D Array of quantum dots on a carbon nanotube

Copyright ACS 2006
Copyright ACS 2006
Copyright ACS 2007
Copyright ACS 2009
Clathrin mediated endocytosis of quantum dots: Colocalization of quantum dots (red)
conjugated with a peptide (allatostatin) and quantum dots (green) conjugated with  clathrin
antibody in human ovarian epidermoid carcinoma cells
Copyright ACS 2009
Copyright ACS 2009
Copyright ACS 2009
Copyright ACS 2009
Copyright ACS 2009

Quantum Dot-DNA

Quantum Dot


pDNA Labeled with Quantum Dots

Nano-Architect: Arranging Nanoparticles

Copyright ACS 2007
Copyright ACS 2008
Copyright ACS 2008
Copyright ACS 2009
Human ovarian epidermoid carcinoma cells
labeled with CdSe-ZnS quantum dots (red)
and Syto16 dye (green)
Quantum dot-peptide
conjugates in 3T3 cells
Quantum dot on the
surface of A431 cells




S. Yamashita, M. Hamada, S. Nakanishi, H. Saito, Y. Nosaka, S. Wakida, V. Biju,Auger Ionization Beats Photo-Oxidation of Semiconductor Quantum Dots: Extended Stability of Single-Molecule Photoluminescence

Angew. Chem. Int. Ed., 54, 3892-3896 (2015) (Featured as Frontispiece Article)

Angew. Chem. 127, 3964– 3968 (2015)


M. Hamada, N. Takanokoshi, K. Motazaki, Q. Feng, N. Murase, S. Wakida, S. Nakanishi, V. Biju, “In Situ Photochemical Surface Passivation of CdSe/ZnS Quantum Dots for Quantitative Light Emission and Enhanced Photocurrent Response in Solar Cells”, J. Phys. Chem. C 118, 2178–2186 (2014). (Featured on the cover)


V. Biju, “Chemical modifications and bioconjugate reactions of nanomaterials for sensing, imaging, drug delivery and therapy”, Chem. Soc. Rev. 43, 744-764 (2014). (Featured on the cover)


Shibu, E. S., Hamada, M., Nakanishi, S., Wakida, S., Biju, V., “Photoluminescence of CdSe and CdSe/ZnS Quantum Dots: Modifications for Making the Invisible Visible at Ensemble and Single-molecule Levels”, Coord. Chem. Rev., 263-264, 2-12 (2014). (Invited thematic article)


Shibu, E. S.; Sugino, S.; Ono, K.; Saito, H.; Nishioka, A.; Yamamura, S.; Sawada, M.; Nosaka, Y.; Biju, V.Singlet-oxygen-sensitizing near-infrared-fluorescent multimodal nanoparticles”, Angew. Chem. Int. Ed., 52, 10559 –10563, 2013.


Shibu, E. S., Ono, K., Sugino, S., Nishioka, A., Yasuda, A., Shigeri, Y., Wakida, S., Sawada, M., Biju, V., “Photouncaging nanoparticles for MRI and fluorescence imaging in vitro and in vivo”, ACS Nano, 7, 9881-9859 (2013).


Jones, P.; Sugino, S.; Yamamura, S.; Lacy, F.; Biju, V.Impairments of cells and genomic DNA by environmentally transformed engineered nanomaterials”, Nanoscale, 5,  9511-9516, 2013. (Featured on the cover)


Shibu, E. S.; Hamada, M.; Murase, N.; Biju, V.Nanomaterials formulations for photothermal and photodynamic therapy of cancer”, J. Photochem. Photobiol. C: Photochem. Rev., 15, 53-72, 2013. (top 25 hottest articles in Science Direct)


V. Biju, A. Anas, H. Akita, E. S. Shibu, T. Itoh, H. Harashima, M. Ishikawa

FRET from quantum dots to photodecompose undesired acceptors and report the condensation and decondensation of plasmid DNA

ACS Nano, 6, 3776-3788, 2012.


E. S. Shibu, A. Sonoda, Z. Tao, Q. Feng, A. Furube, S. Masuo, L. Wang, N. Tamai, M. Ishikawa, V. Biju

“Photofabrication of fullerene-shelled quantum dots supramolecular nanoparticles for solar energy harvesting”

ACS Nano, 6, 1601-1608, 2012.


V. Biju, T. Itoh, M. Ishikawa

Delivering quantum dots to cells: bioconjugated quantum dots for extracellular and intracellular imaging

Chem. Soc. Rev. 39, 3031-3056,2010 (Highlighted in Chemical Biology, Royal Society)

M. Hamada, S. Nakanishi, T. Itoh, M. Ishikawa, V. Biju

 “Blinking suppression in CdSe/ZnS single quantum dots by TiO2 nanoparticles

ACS Nano, 4, 4445-4454, 2010

N. Kawashima, K. Nakayama, K. Itoh, T. Itoh, M. Ishikawa, V. Biju

“Reversible dimerization of EGFR revealed by single-molecule fluorescence imaging using quantum dots”

Chem. Eur. J., 16, 1186-1192, 2010. (Featured on the Cover)

V. Biju, M. Sathish, R. V. Omkumar, A. Anas, M. Ishikawa

Bioconjugated Quantum Dots for Cancer Research: Present Status, Prospects and Remaining Issues

Biotechnol. Adv., 28, 199-213, 2010.

A. Anas, T. Okuda, N. Kawashima, K. Nakayama, T. Itoh, M. Ishikawa, V. Biju

“Clathrin-mediated endocytosis of quantum dot-peptide conjugates in living cells”

ACS Nano, 3, 2419-2429, 2009. (Highlighted Science and Technology News in C&EN)

V. Biju, T. Itoh, A. Anas, S. Sujith, M. Ishikawa

Semiconductor Quantum Dots and Metal Nanoparticles: Syntheses, Optical Properties, and Biological Applications”

Anal. Bioanal. Chem., 391, 2469–2495, 2008. (top 10 cited article in the journal)

A. Anas, H. Akita, H. Harashima, M. Ishikawa, V. Biju

Photosensitized breakage and damage of DNA by CdSe-ZnS quantum dots

J. Phys. Chem. B, 112, 10005-10011, 2008 (Featured on the Front Cover).

R. Kanemoto, A. Anas, Y. Matsumoto, R. Ueji, T. Itoh, Y. Baba, S. Nakanishi, M. Ishikawa, V. Biju

Relations between dewetting of polymer thin films and phase-separation of encompassed quantum dots

J. Phys. Chem. C, 112, 9312-9319, 2008 (Featured on the Front Cover).


Y. Matsumoto, R. Kanemoto, T. Itoh, S. Nakanishi, M. Ishikawa, V. Biju

“Photoluminescence quenching and intensity fluctuations of CdSe-ZnS quantum dots on an Ag nanoparticle film”

J. Phys. Chem. C, 112, 1345-1350, 2008 (Featured on the Front Cover).


V. Biju, R. Kanemoto, Y. Matsumoto, S. Ishii, T. Itoh, S. Nakanishi, Y. Baba, M. Ishikawa

“Photoinduced photoluminescence variations of CdSe quantum dots in polymer solutions”

J. Phys. Chem. C, 111, 7924-7932, 2007 (Featured on the Front Cover).

V. Biju, Y. Makita, A. Sonoda, H. Yokoyama, Y. Baba, M. Ishikawa

“Temperature-sensitive photoluminescence of CdSe quantum dot clusters”

J. Phys. Chem. B, 109, 13899-13905, 2005  (Featured on the Front Cover).

V. Biju, M. Micic, D. Hu, H. P. Lu

“Intermittent single-molecule interfacial electron transfer dynamics”

J. Am. Chem. Soc., 126, 9374-9381, 2004.





“Nanomaterials Engineered for Single-molecule Detections and Multimodal Bioimaging”, 3rd International Workshop and Summer course, National Chiao Tung University, Hshichu, Taiwan, 2014.7.10 (Invited Lecture)

“Light and Materials in the Pursuit of Bilateral Relations”, 2014 Korea-Japan Frontier Photoscience Symposium, Seoul, Republic of Korea, 2014.6.22 (Keynote Lecture)

“On the Optical Pathway of Nanomaterials to Life Science: Pitfalls, Prospects and the Roles of Cell Surface Receptors”, Optics 14, NIT Calicut, India, 2014.3.20 (Invited Lecture)

“Prospects and Pitfalls in the Applications of Nanomaterials for Energy and Life Innovations”, Mahatma Gandhi University, Kottayam, India 2014.3.21 (Invited Lecture)

“Nanomaterials formulations for biomedical applications”, Nanotech 2014, Tokyo Big Sight, 2014.1.30 (Invited Lecture)

“Nanomaterials for Multiplexed Fluorescence Imaging”, 4th Asian Spectroscopy Conference, Nanyang Technological University, Singapore, 2013.12.17 (Invited Lecture).

“Engineered nanomaterials: Are they potential agents for biological applications or potentiators of toxicity?”, Symposium on "Emerging Materials for Health, Environment and Safety" organized at the Embassy of Indian, Tokyo, Japan, 2013.10.11 (Plenary Lecture).

Emerging trends in nanomedicine and bioimaging: theranostics go in vitro and in vivo", Sanker’s Institute of Medical Science, Kollam, Kerala, India, 2013.8.2 (Public Address)

“The Changing Life and Ceaseless Diseases”, SNDP Hall, Adichanalloor, India, 2013.8.22 (Public Address)

“Photoresponsive nanomaterials for bioimaging”, 2013 International Conference on Photochemistry, Katholike University of Leuven, Belgium, 2013.07.24.

“International Conference on Materials for Advanced Technologies”, Suntech Convention Center, Singapore, 2013.07.04 (Invited Lecture).

Multifunctional Engineered Nanomaterials: Bioimaging Applications Vs Toxicity”, International Workshop on Photonics of Functional Nanomaterials, City University of Hong Kong, 2013.05.09 (Invited Lecture).

“Bioengineered Nanomaterials: from Bioimaging to Nanotoxicity”, International conference on Nanoscience and Nanotechnology, SRM University, India, 2013.03.18 (Plenary Lecture).

“Quantum Dots for Bioimaging”, Seminar of the DST Unit of Nanoscience, IIT Madras, India, 2013.3.17 (Invited Lecture)

“Optical Properties, Bioimaging Applications and Cytotoxicity of Engineered Nanomaterials”, the University of Strathclyde, Glasgow, UK, 2013.02.05 (Invited Lecture).

“Nanomaterials Formulations for Photoinduced Electron Transfer”, DST-JSPS Indo-Japan Bilateral Seminar, Takamatsu, Japan, 2012.10.16.

“Energy and electron transfer in quantum dots based systems”, RCAS-TNNA International Symposium, Academia Sinica, Taipei, Taiwan 2012.10 (Invited Lecture)

“Semiconductor quantum dots for single-molecule imaging”, Young Lectureship during the Annual Meeting of the Chemical Society of Japan, Yokohama, Japan 2011.3 (Lectureship Price Lecture)

“Single semiconductor quantum dots: From blinking suppression to lighting up biomolecular functions” Young Scientist Award for Asian and Oceanian Photochemist, Wellington, New Zealand, 2010.11 (Award Lecture)

“Optimization of optical properties of colloidal quantum dots for biological applications” Asian International Symposium of the Chemical Society of Japan, Osaka, Japan, 2010.3 (Keynote Lecture for the Distinguished Lectureship Award by the Chemical Society of Japan)

“Probing biophysical functions in vitro using flow cytometry and single-molecule microscopy” Academia Sinica, Taipei, Taiwan, 2009.10 (Invited Lecture)

“Bioconjugated quantum dots for probing biophysical dynamics in living cells” The 2nd Laser Bio/Nano Science Seminar, National Chiao Tung University, Taipei, Taiwan, 2009.10 (Invited Lecture)

“Bioconjugated quantum dots for imaging of cellular processes”, Kerala University, India, 2009.08 (Invited Lecture)

“Bioconjugated quantum dots: Candidates for intracellular delivery for probing intracellular signaling” Biotechnology Seminar, Rajiv Gandhi Center for Biotechnology, Trivandrum, India (Invited Lecture)

“Endless scope of Nanobiotechnology” Science Seminar, Public Library, Kerala, India 2009.08 (Invited Lecture)

“Semiconductor Quantum Dots: Syntheses, Photoluminescence Properties, and Prospective of CdSe and CdSe-ZnS Nanocrystals” Tokushima, Japan, 2008.01 (Invited Lecture)

“A Decade of Semiconductor Quantum Dots in Biology: Bioconjugation, In Vitro and In Vivo Imaging, and FRET” Kerala University, Trivandrum, India, 2008.03 (Invited Lecture)

“Semiconductor Quantum Dots and Metal Nanoparticles: Optical Properties for Biological Applications” Kerala University, Trivandrum, India, 2008.03 (Invited Lecture)

“Microscopy and Microspectroscopy in Nanobioimaging: Instrumentation and Applications of AFM, TIRFM, and FLIM” Kerala University, Trivandrum, India, 2008.03 (Invited Talk)

“Close-packing of colloidal quantum dots: Photoluminescence properties of linear and spheroid assemblies of quantum dots”American Chemical Society Meeting, San Francisco, USA, 2006.09 (Special Symposium Lecture in the honor of Professor Gabor Somorjai).

“Nanoscale Materials in Bioscience” Colloquium on Nanoscale Science and Arthropod Bioresources, Mascot Hotel, Trivandrum, India 2006.12 (Invited Lecture)

“Millisecond luminescence intermittencies of single CdSe quantum dots” Kick off Meeting of the Photonics forum, AIST Tsukuba, Japan, 2005.02 (Invited Lecture)



I am interested in modern nano and bionano tools and technologies based on multimodal analyses of a wide spectrum of molecules, nanomaterials and living systems for the advancement of health technology. The main research area I am focusing at is the use of bioconjugated quantum dots and fluorescent/fluorescent-magnetic nonomaterials, and spectroscopy and microscopy, in particular, atomic force microscopy, fluorescence microscopy and time-resolved fluorescence spectroscopy, for optimization of relaxation processes in highly excited semiconductor quantum dots, and unraveling of subcellular processes and the structures and functioning of biomolecules. For example, currently, I am investigating the role of Auger relaxation on the physico-chemical stability of single quantum dots, relaxation mechanism underlying the activation of cell signaling molecules, and intracellular delivery of nanobioconjugates of imaging and therapeutic potentials. Also, I am developing novel nano/bionanomaterials, and optimizing the optical properties of single semiconductor nanoparticles for advanced bioanalysis and nanobiotechnology.