本文へスキップ

TEL.029-861-3408

〒305-8586 茨城県つくば市梅園1-1-1中央第2

研究業績achievements

 主要論文(2001〜2012)

  1. Ogura T (2012)“ High-Contrast Observation of Unstained Proteins and Viruses by Scanning Electron Microscopy.”, PLoS ONE 7(10): e46904.
  2. Toshihiko Ogura, "Direct observation of the inner structure of unstained atmospheric cells by low-energy electrons", Meas. Sci. Technol.Vol. 23, 085402 (2012)
  3. Ogura T, " Three-Dimensional X-ray Observation of Atmospheric Biological Samples by Linear-Array Scanning-Electron Generation X-ray Microscope System." , PLoS ONE, 23 Jun (2011)
  4. Venruti, E., Mio, K., Nishi, M., Ogura, T., Moriya, T., Pitt, S.J., Okuda, K., Kakizawa, S., Sitsapesan, R., Sato, C. and Takeshima, H., “Mitsugumin 23 forms a massive bowl-shaped assembly and cation-conducting channel”, Biochemistry, Vol. 50, pp.2623-2632 (2011)
  5.  * Ogura, T., “High-resolution X-ray observation of unstained samples by a newly developed SGXM”, Nanotechnology, Vol .21, 295501 (2010)
  6.  * Ogura, T., Tong, I.K., Mio, K., Maruyama, Y., Kurokawa, H., Sato, C. and Yamamoto M, “Keap1 is a forked-stem dimmer structure with two large spheres enclosing the intervening, double glycine repeat, and C-terminal domains”, Proc. Natl. Acad. Sci USA, Vol.107, pp.2842-2847 (2010)
  7. * Ogura, T., “Direct observation of unstained wet biological samples by scanning-electron generation X-ray microscopy”, Biochem. Biophys. Res. Commun., Vol.391, pp.198-202 (2010)
  8. Nishiyama, H., Suga, M., Ogura, T., Maruyama, Y., Koizumi, M., Mio, K., Kitamura, S. and Sato, C., “Atmosheric scanning electron microscope observes cells and tissues in open medium through silicon nitride film”, J. Struct. Biol., Vol.169, pp.438-449 (2010)
  9. * Ogura, T., “Measurement of the unstained biological sample by a novel scanning electron generation X-ray microscope based on SEM”, Biochem. Biophys. Res. Commun., Vol.385, pp.624-629 (2009)
  10. Maruyama, Y., Ogura, T., Mio, K., Kiyonaka, S., Kato, K., Mori, Y. and Sato, C., “Tetrameric Orai1 is a Teardrop-shaped molecule with a long, tapered cytoplasmic domain”, J. Biol. Chem., Vol.169, pp.438-449 (2009)
  11. * Ogura, T., “Analyzing indirect secondary electron contrast of unstained bacteriophage T4 based on SEM images and Monte Carlo simulations”, Biochem. Biophys. Res. Commun., Vol.380, pp.254-259 (2009)
  12. Mio, K., Ogura, T., Yamamoto, T., Hiroaki, Y., Fujiyoshi, Y., Kubo, Y. and Sato, C., “Reconstruction of the P2X2 receptor reveals a vase-shaped structure with lateral tunnels above the membrane”, Structure, 17, pp.266-275 (2009)
  13. Omura, A., Matsuzaki, T., Mio, K., Ogura, T., Yamamoto, M., Fujita, A., Okawa, K., Kitayama, H., Takahashi, C., Sato, C. and Noda, M., “RECK forms cowbell-shaped dimers and inhibits matrix metalloproteinase-catalyzed cleavage of fibronectin”, J. Biol. Chem., Vol.284, pp.3461-3469 (2009)
  14. * Ogura, T., “A high contrast method of unstained biological samples under a thin carbon film by scanning electron microscopy”, Biochem. Biophys. Res. Commun., Vol.377, pp.79-84 (2008)
  15. Mio, K., Ogura, T., Mio, M., Shimizu, H., Hwang, T.C., Sato, C. and Sohma, Y., “Three-dimensional reconstruction of human cystic fibrosis transmembrane regulator chloride channel revealed an ellipsoidal structure with orifices beneath the putative transmenbrane domain”, J. Biol. Chem., Vol.283, pp.30300-30310 (2008)
  16. Mio, K., Ogura, T. ,Sato, C., “Structure of six-transmembrane cation channels revealed by single-particle analysis from electron microscopic images”, J. Sync. Radiat., Vol.283, pp.30300-30310 (2008)
  17. Yazawa, M., Ferrante, C., Feng, J., Mio, K., Ogura, T., Zhang, M., Lin, P.H., Pan, Z., Komazaki, S., Kato, K., Nishi, M., Zhao, X., Weisleder, N., Sato, C., Ma, J. and Takeshima, H., “TRIC channels are essential for Ca2+ handling in intracellular stores”, Nature, Vol.448, pp.78-82 (2007)
  18. Maruyama, Y., Ogura, T., Mio, K., Kiyonaka, S., Kato, K., Mori, Y. and Sato, C., “Three-dimensional reconstruction using transmission electron microscopy reveals a swollen, Bell-shaped structure of transient receptor potential melastatin type2 cation channel”, J. Biol. Chem., Vol.282, pp.36961-36970 (2007)
  19. Mio, K., Ogura, T., Kiyonaka, S., Hiroaki, Y., Tanimura, Y., Fujiyoshi, Y., Mori, Y. and Sato, C., “The TRPC3 channel has a large internal chamber surrounded by signal sensing antennas”, J. Mol. Biol., Vol.367, pp.373-383 (2007)
  20. * Ogura, T., Mio, K., Hayashi, I., Miyashita, H., Fukuda, R., Kopan, R., Kodama, T., Hamakubo, T., Iwatsubo, T., Tomita, T. and Sato, C., “Three-dimensional structure of the γ-secretase complex”, Biochem. Biophys. Res. Commun., Vol.343, pp.525-534 (2006)
  21. * Ogura, T. and Sato, C., “A fully automatic 3D reconstruction method using simulated annealing enables accurate posterioric angular assignment of protein projections”, J. Struct. Biol., Vol.156, pp.371-386 (2006)
  22. Mio, K., Kubo, Y., Ogura, T., Yamamoto, T. and Sato, C., “Visualization of trimeric P2X2 receptor with crown capped extracellular domain”, Biochem. Biophys. Res. Commun., Vol.337, pp.998-1005 (2005)
  23.  Mio, K., Ogura, T., Hara, Y., Mori, Y. and Sato, C., “The non-selective cation-permeable channel TRPC3 is a tetrahedron with a cap on the large cytoplasmic end”, Biochem. Biophys. Res. Commun., Vol.333, pp.768-777 (2005)
  24. * Ogura, T. and Sato, C., “Auto-accumulation method using simulated annealing enables fully automatic particle pickup completely free from a matching template or learning data”, J. Struct. Biol., 146, pp.344-358 (2004)
  25. * Ogura, T. and Sato, C., “Automatic particle pickup method using a neural network has high accuracy by applying an initial weight derived from eigenimages: a new reference free method for single-particle analysis”, J. Struct. Biol., 145, pp. 63-75 (2004)
  26.  Sato, C., Hamada, K., Ogura, T., Miyazawa, A., Iwasaki, K., Hiroaki, Y., Tani, K., Terauchi, A., Fujiyoshi, Y. and Mikoshiba, K., “Inositol 1,4,5-trisphosphate receptor contains multiple cavities and L-shaped ligand-binding domains”, J. Mol. Biol., 336, pp. 155-164 (2004)
  27. * Ogura, T., Iwasaki, K. and Sato, C., “Topology representing network enables highly-accurate classification of Cryo electron micrograph of protein without masking”, J. Struct. Biol., 143, pp.185-200 (2003)
  28. * Ogura, T., Satoh, T.O., Usui, S. and Yamada, M., “A simulation analysis on mechanisms of damped oscillation in retinal rod photoreceptor cells”, Vision Res., 43, pp.2109-2028 (2003)
  29. * Ogura, T. and Sato, C., “An automatic particle pickup method using a neural network applicable to low-contrelectron micrographs”, J. Struct. Biol., 136, pp. 227-238 (2001)
店舗イメージ

小椋俊彦 Ogura Toshihiko
独立行政法人産業技術総合研究所
バイオメディカル研究部門

構造生理研究グループ


連絡先:

〒305-8586
茨城県つくば市梅園1-1-1中央第2
TEL:029-861-3408
FAX:029-861-5065
Mail:t-ogura@aist.go.jp