Shigeyuki Kakizawa's Homepage

産総研
last updated 2023.9.14
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柿澤 茂行（Shigeyuki Kakizawa）
国立研究開発法人産業技術総合研究所
生物プロセス研究部門（ウェブサイト）
生物共生進化機構研究グループ　主任研究員
〒305-8568 茨城県つくば市梅園1-1-1　中央第6
E-mail：s.kakizawa*aist.go.jp（*を@に変更して使用してください。）
学歴
  • 2004年3月 東京大学 大学院 新領域創成科学研究科 先端生命科学専攻 博士課程 修了
  • 2004年3月 博士（生命科学）
経歴
  • 2011年～2013年　産業技術総合研究所　生物プロセス研究部門　研究員
  • 2013年～現在　産業技術総合研究所　生物プロセス研究部門　主任研究員
研究実績

Research Map

Google scholar citations

論文・総説・書籍

  • 1. 水谷雅希, 古賀隆一, 深津武馬, 柿澤茂行 (2023) 細菌の全ゲノムクローニングと未培養細菌への応用. in 未培養微生物研究の最新動向. ed. 青柳秀紀. pp 82-91.
  • 2. 柿澤茂行 (2023) 細菌の全ゲノム合成. 実験医学, 41(3), 432-436.
  • 3. Kakizawa S (2023) Development and utilization of Mycoplasma minimal cells. J. Jpn. Soc. Extremophiles 21(2).
  • 4. Kiyama H, Kakizawa S, Sasajima Y, Tahara YO, Miyata M. (2022) Reconstitution of a minimal motility system based on Spiroplasma swimming by two bacterial actins in a synthetic minimal bacterium. Sci. Adv. 8,eabo7490. DOI:10.1126/sciadv.abo7490
  • 5. Kakizawa S, Hosokawa T, Oguchi K, Miyakoshi K, Fukatsu T. (2022) Spiroplasma as facultative bacterial symbionts of stinkbugs. Front Microbiol. 13. doi: 10.3389/fmicb.2022.1044771.
  • 6. Nishiumi F, Kawai Y, Nakura Y, Yoshimura M, Wu HN, Hamaguchi M, Kakizawa S, Suzuki Y, Glass JI, Yanagihara I. (2021) Blockade of endoplasmic reticulum stress-induced cell death by Ureaplasma parvum vacuolating factor. Cell Microbiol:e13392. doi: 10.1111/cmi.13392.
  • 7. 柿澤 茂行 (2021)．人工生命？ 人工のゲノムをもった細菌．生物工学会誌，99(6), 303. https://doi.org/10.34565/seibutsukogaku.99.6_303 8. 柿澤 茂行 (2020)．細菌のゲノム編集．日本ゲノム微生物学会誌, 22, 6-8.
  • 9. 五十嵐 健輔, 柿澤 茂行 (2020)．硫化鉱物による化学進化と鉄イオウタンパク質の誕生．Journal of Geography (Chigaku Zasshi), 129(6), 853-870. https://doi.org/10.5026/jgeography.129.853
  • 10. 馬場知哉・柿澤茂行・森　宙史・車　兪澈・黒川　顕・大島　拓 (2020)．最小ゲノム —細胞が生きるために必要な遺伝子数はいくつか—．Journal of Geography (Chigaku Zasshi), 129(6), 805-824. https://doi.org/10.5026/jgeography.129.805
  • 11. 篠田 健太郎, 齋藤 宏輝, 柿澤 茂行, 明渡 純 (2020) 新型コロナウイルスの伝搬過程と溶射プロセスの比較．日本溶射学会誌「溶射」．2020 年 57 巻 4 号 p. 197-206. https://doi.org/10.11330/jtss.57.197
  • 12. Rao GP, Manimekalai R, Kumar M, Ranebennur H, Kakizawa S, Bertaccini A. Host Metabolic Interaction and Perspectives in Phytoplasma Research. Phytoplasmas: Plant Pathogenic Bacteria - III，: Springer Nature; 2019.
  • 13. Kakizawa S, Nijo T. (2019) ファイトプラズマの宿主特異性と遺伝子発現（Host specificity and gene expression of phytoplasma）. AGRICULTURAL BIOTECHNOLOGY 3(12):21-6.
  • 14. Kakizawa S. (2019) A Multiplex-PCR Method for Diagnosis of AY-Group Phytoplasmas. Methods Mol Biol 1875:143-9. doi: 10.1007/978-1-4939-8837-2_11.
  • 15. Mariscal AM, Kakizawa S, Hsu JY, Tanaka K, Gonzalez-Gonzalez L, Broto A, Querol E, Lluch-Senar M, Pinero-Lambea C, Sun L, Weyman PD, Wise KS, Merryman C, Tse G, Moore AJ, Hutchison CA, 3rd, Smith HO, Tomita M, Venter JC, Glass JI, Pinol J, Suzuki Y. (2018) Tuning Gene Activity by Inducible and Targeted Regulation of Gene Expression in Minimal Bacterial Cells. ACS Synth Biol 7(6):1538-52. doi: 10.1021/acssynbio.8b00028.
  • 16. Kakizawa S. (2018) Phytoplasma: lazy bacterial pathogens that alter plant morphology (ファイトプラズマ：植物の形を変える怠け者細菌). 日本微生物生態学会誌 33(2):12-8.
  • 17. Kakizawa S. ファイトプラズマ感染の診断. In: 泉川欣一, 賀来満夫, 神谷茂, 佐々木次雄, 難波成任, 宮田真人, editors. 最新マイコプラズマ学: 近代出版; 2016. p. 72-6.
  • 18. Bertaccini A, Oshima K, Kakizawa S, Duduk B, Namba S. Dissecting the multi-faceted mechanisms that drive leafhopper host-phytoplasma specificity. In: Brown JK, editor. Vector-Mediated Transmission of Plant Pathogens. St Paul MN: APS Press; 2016. p. 21-8.
  • 19. Kakizawa S, Yoneda Y. (2015) The role of genome sequencing in phytoplasma research. Phytopathogenic Mollicutes 5(1):19-24. doi: 10.5958/2249-4677.2015.00058.4.
  • 20. Kakizawa S. (2015) Methods for cloning of bacterial genomes in yeast. J Mycoplasmology 41:4-6.
  • 21. Kakizawa S, Makino A, Ishii Y, Tamaki H, Kamagata Y. (2014) Draft Genome Sequence of "Candidatus Phytoplasma asteris" Strain OY-V, an Unculturable Plant-Pathogenic Bacterium. Genome Announc 2(5):e00944-14. . doi: 10.1128/genomeA.00944-14.
  • 22. Kakizawa S, Kamagata Y. (2014) A multiplex-PCR method for strain identification and detailed phylogenetic analysis of AY-group phytoplasmas. Plant Disease 98(3):299-305. doi: 10.1094/PDIS-03-13-0216-RE.
  • 23. Ishii Y, Matsuura Y, Kakizawa S, Nikoh N, Fukatsu T. (2013) Diversity of bacterial endosymbionts associated with Macrosteles leafhoppers vectoring phytopathogenic phytoplasmas. Appl Environ Microbiol 79(16):5013-22.
  • 24. Ishii Y, Kakizawa S, Oshima K. (2013) New ex vivo reporter assay system reveals that sigma factors of an unculturable pathogen control gene regulation involved in the host switching between insects and plants. Microbiologyopen 2(4):553-65.
  • 25. Oshima K, Ishii Y, Kakizawa S, Sugawara K, Neriya Y, Himeno M, Minato N, Miura C, Shiraishi T, Yamaji Y, Namba S. (2011) Dramatic transcriptional changes in an intracellular parasite enable host switching between plant and insect. PLoS One 6(8):e23242.
  • 26. Neriya Y, Sugawara K, Maejima K, Hashimoto M, Komatsu K, Minato N, Miura C, Kakizawa S, Yamaji Y, Oshima K, Namba S. (2011) Cloning, expression analysis, and sequence diversity of genes encoding two different immunodominant membrane proteins in poinsettia branch-inducing phytoplasma (PoiBI). FEMS Microbiol Lett 324(1):38-47.
  • 27. Mitrovic J, Kakizawa S, Duduk B, Oshima K, Namba S, Bertaccini A. (2011) The groEL gene as an additional marker for finer differentiation of 'Candidatus Phytoplasma asteris'-related strains. Ann Appl Biol 159(1):41-8.
  • 28. Kawanishi T, Shiraishi T, Okano Y, Sugawara K, Hashimoto M, Maejima K, Komatsu K, Kakizawa S, Yamaji Y, Hamamoto H, Oshima K, Namba S. (2011) New detection systems of bacteria using highly selective media designed by SMART: selective medium-design algorithm restricted by two constraints. PLoS One 6(1):e16512.
  • 29. Kakizawa S, Hoshi A, Ishii Y, Kojima N, Sugawara K, Okano Y, Maejima K, Oshima K, Namba S. Unique features of a phytoplasma genome and its membrane proteins involved in host specificity. In: Wolpert T, Shiraishi T, Collmer A, Akimitsu K, Glazebrook J, editors. Genome-enabled analysis of plant-pathogen interactions Minnesota: APS press; 2011. p. 103-10.
  • 30. Himeno M, Neriya Y, Minato N, Miura C, Sugawara K, Ishii Y, Yamaji Y, Kakizawa S, Oshima K, Namba S. (2011) Unique morphological changes in plant pathogenic phytoplasma-infected petunia flowers are related to transcriptional regulation of floral homeotic genes in an organ-specific manner. Plant J 67(6):971-9.
  • 31. Kakizawa S, Oshima K, Namba S. Functional genomics of phytoplasmas. In: Weintraub PG, Jones P, editors. Phytoplasmas: genome, plant hosts and vectors. Oxfordshire: CABI; 2010. p. 37-50.
  • 32. Kakizawa S, Oshima K, Ishii Y, Hoshi A, Maejima K, Jung HY, Yamaji Y, Namba S. (2009) Cloning of immunodominant membrane protein genes of phytoplasmas and their in planta expression. FEMS Microbiol Lett 293(1):92-101.
  • 33. Ishii Y, Oshima K, Kakizawa S, Hoshi A, Maejima K, Kagiwada S, Yamaji Y, Namba S. (2009) Process of reductive evolution during 10 years in plasmids of a non-insect-transmissible phytoplasma. Gene 446(2):51-7.
  • 34. Ishii Y, Kakizawa S, Hoshi A, Maejima K, Kagiwada S, Yamaji Y, Oshima K, Namba S. (2009) In the non-insect-transmissible line of onion yellows phytoplasma (OY-NIM), the plasmid-encoded transmembrane protein ORF3 lacks the major promoter region. Microbiology 155(Pt 6):2058-67.
  • 35. Hoshi A, Oshima K, Kakizawa S, Ishii Y, Ozeki J, Hashimoto M, Komatsu K, Kagiwada S, Yamaji Y, Namba S. (2009) A unique virulence factor for proliferation and dwarfism in plants identified from a phytopathogenic bacterium. Proc Natl Acad Sci U S A 106(15):6416-21. doi: 10.1073/pnas.0813038106.
  • 36. Maejima K, Himeno M, Komatsu K, Kakizawa S, Yamaji Y, Hamamoto H, Namba S. (2008) Complete nucleotide sequence of a new double-stranded RNA virus from the rice blast fungus, Magnaporthe oryzae. Arch Virol 153(2):389-91.
  • 37. Hogenhout SA, Oshima K, Ammar E-D, Kakizawa S, Kingdom HN, Namba S. (2008) Phytoplasmas: Bacteria that manipulate plants and insects. Mol Plant Pathol 9(4):403-23.
  • 38. Arashida R, Kakizawa S, Ishii Y, Hoshi A, Jung HY, Kagiwada S, Yamaji Y, Oshima K, Namba S. (2008) Cloning and characterization of the antigenic membrane protein (Amp) gene and in situ detection of Amp from malformed flowers infected with Japanese hydrangea phyllody phytoplasma. Phytopathology 98(7):769-75.
  • 39. Arashida R, Kakizawa S, Hoshi A, Ishii Y, Jung HY, Kagiwada S, Yamaji Y, Oshima K, Namba S. (2008) Heterogeneic dynamics of the structures of multiple gene clusters in two pathogenetically different lines originating from the same phytoplasma. DNA Cell Biol 27(4):209-17.
  • 40. Oshima K, Kakizawa S, Arashida R, Ishii Y, Hoshi A, Hayashi Y, Kagiwada S, Namba S. (2007) Presence of two glycolytic gene clusters in a severe pathogenic line of Candidatus Phytoplasma asteris. Mol Plant Pathol 8(4):481-9.
  • 41. Suzuki S, Oshima K, Kakizawa S, Arashida R, Jung HY, Yamaji Y, Nishigawa H, Ugaki M, Namba S. (2006) Interaction between the membrane protein of a pathogen and insect microfilament complex determines insect-vector specificity. Proc Natl Acad Sci U S A 103(11):4252-7.
  • 42. Kakizawa S, Oshima K, Namba S. (2006) Diversity and functional importance of phytoplasma membrane proteins. Trends Microbiol 14(6):254-6.
  • 43. Kakizawa S, Oshima K, Jung HY, Suzuki S, Nishigawa H, Arashida R, Miyata S, Ugaki M, Kishino H, Namba S. (2006) Positive selection acting on a surface membrane protein of the plant-pathogenic phytoplasmas. J Bacteriol 188(9):3424-8.
  • 44. Wei W, Kakizawa S, Suzuki S, Jung HY, Nishigawa H, Miyata S, Oshima K, Ugaki M, Hibi T, Namba S. (2004) In planta dynamic analysis of onion yellows phytoplasma using localized inoculation by insect transmission. Phytopathology 94(3):244-50.
  • 45. Wei W, Kakizawa S, Jung HY, Suzuki S, Tanaka M, Nishigawa H, Miyata S, Oshima K, Ugaki M, Hibi T, Namba S. (2004) An antibody against the SecA membrane protein of one phytoplasma reacts with those of phylogenetically different phytoplasmas. Phytopathology 94(7):683-6.
  • 46. Oshima K, Kakizawa S, Nishigawa H, Jung HY, Wei W, Suzuki S, Arashida R, Nakata D, Miyata S, Ugaki M, Namba S. (2004) Reductive evolution suggested from the complete genome sequence of a plant-pathogenic phytoplasma. Nature Genet 36(1):27-9.
  • 47. Kakizawa S, Oshima K, Nishigawa H, Jung HY, Wei W, Suzuki S, Tanaka M, Miyata S, Ugaki M, Namba S. (2004) Secretion of immunodominant membrane protein from onion yellows phytoplasma through the Sec protein-translocation system in Escherichia coli. Microbiology 150(Pt 1):135-42.
  • 48. Miyata S, Oshima K, Kakizawa S, Nishigawa H, Jung HY, Kuboyama T, Ugaki M, Namba S. (2003) Two different thymidylate kinase gene homologues, including one that has catalytic activity, are encoded in the onion yellows phytoplasma genome. Microbiology 149(Pt 8):2243-50.
  • 49. Jung HY, Sawayanagi T, Wongkaew P, Kakizawa S, Nishigawa H, Wei W, Oshima K, Miyata S, Ugaki M, Hibi T, Namba S. (2003) "Candidatus Phytoplasma oryzae", a novel phytoplasma taxon associated with rice yellow dwarf disease. Int J Syst Evol Microbiol 53(Pt 6):1925-9.
  • 50. Jung HY, Sawayanagi T, Kakizawa S, Nishigawa H, Wei W, Oshima K, Miyata S, Ugaki M, Hibi T, Namba S. (2003) 'Candidatus phytoplasma ziziphi', a novel phytoplasma taxon associated with jujube witches'-broom disease. Int J Syst Evol Microbiol 53(Pt 4):1037-41.
  • 51. Jung HY, Miyata S, Oshima K, Kakizawa S, Nishigawa H, Wei W, Suzuki S, Ugaki M, Hibi T, Namba S. (2003) First complete nucleotide sequence and heterologous gene organization of the two rRNA operons in the phytoplasma genome. DNA Cell Biol 22(3):209-15.
  • 52. Oshima K, Miyata S, Sawayanagi T, Kakizawa S, Nishigawa H, Jung H, Furuki K, Yanazaki M, Suzuki S, Wei W, Kuboyama T, Ugaki M, Namba S. (2002) Minimal set of metabolic pathways suggested from the genome of onion yellows phytoplasma. J Gen Plant Pathol 68:225-36.
  • 53. Nishigawa H, Oshima K, Kakizawa S, Jung HY, Kuboyama T, Miyata S, Ugaki M, Namba S. (2002) Evidence of intermolecular recombination between extrachromosomal DNAs in phytoplasma: a trigger for the biological diversity of phytoplasma? Microbiology 148(Pt 5):1389-96.
  • 54. Nishigawa H, Oshima K, Kakizawa S, Jung HY, Kuboyama T, Miyata S, Ugaki M, Namba S. (2002) A plasmid from a non-insect-transmissible line of a phytoplasma lacks two open reading frames that exist in the plasmid from the wild-type line. Gene 298(2):195-201.
  • 55. Miyata S, Furuki K, Oshima K, Sawayanagi T, Nishigawa H, Kakizawa S, Jung HY, Ugaki M, Namba S. (2002) Complete nucleotide sequence of the S10-spc operon of phytoplasma: gene organization and genetic code resemble those of Bacillus subtilis. DNA Cell Biol 21(7):527-34.
  • 56. Jung HY, Sawayanagi T, Kakizawa S, Nishigawa H, Miyata S, Oshima K, Ugaki M, Lee JT, Hibi T, Namba S. (2002) 'Candidatus Phytoplasma castaneae', a novel phytoplasma taxon associated with chestnut witches' broom disease. Int J Syst Evol Microbiol 52(Pt 5):1543-9.
  • 57. Tamakoshi M, Nakano Y, Kakizawa S, Yamagishi A, Oshima T. (2001) Selection of stabilized 3-isopropylmalate dehydrogenase of Saccharomyces cerevisiae using the host-vector system of an extreme thermophile, Thermus thermophilus. Extremophiles 5(1):17-22.
  • 58. Oshima K, Shiomi T, Kuboyama T, Sawayanagi T, Nishigawa H, Kakizawa S, Miyata S, Ugaki M, Namba S. (2001) Isolation and characterization of derivative lines of the onion yellows phytoplasma that do not cause stunting or phloem hyperplasia. Phytopathology 91(11):1024-9.
  • 59. Oshima K, Kakizawa S, Nishigawa H, Kuboyama T, Miyata S, Ugaki M, Namba S. (2001) A plasmid of phytoplasma encodes a unique replication protein having both plasmid- and virus-like domains: clue to viral ancestry or result of virus/plasmid recombination? Virology 285(2):270-7.
  • 60. Kakizawa S, Oshima K, Kuboyama T, Nishigawa H, Jung HY, Sawayanagi T, Tsuchizaki T, Miyata S, Ugaki M, Namba S. (2001) Cloning and expression analysis of Phytoplasma protein translocation genes. Mol Plant Microbe Interact 14(9):1043-50.

特許

  • 特許第3521841号 ファイトプラズマの検出方法. 難波 成任, 宇垣 正志, 宮田 伸一, 大島 研郎, 西川 尚志, 柿澤 茂行