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最近の業績リスト
(1)総説 (2)論文 (3)口頭発表と招待講演 (4)国内外特許


(1)総説
  1. I. Matsui and K. Harata “Implication for buried polar contacts and ion-pairs in hyperthermostable enzymes” (2007) FEBS J., 274, 4012-4022.

  2. 松井郁夫、漆畑祐司、松井えり子 “100℃で働くDNA複製装置”(2006)蛋白質核酸酵素、51巻、9号、p1072-1080.

  3. Y. Shen, X.-F. Tang, E. Matsui, and I. Matsui “Subunit interaction and regulation of activity through terminal domains of the family D DNA polymerase from Pyrococcus horikoshii“ (2004) Biochem. Soci. Trans., 32(Pt 2), 245-249.


(2)論文
  1. Matsui I, Urushibata Y, Shen Y, Matsui E, Yokoyama H “Novel structure of an N-terminal domain that is crucial for the dimeric assembly and DNA-binding of an archaeal DNA polymerase D large subunit from Pyrococcus horikoshii” (2011) FEBS Lett., 585, 452-458.

  2. Yamasaki K, Urushibata Y, Yamasaki T, Arisaka F, and Matsui I. “Solution structure of the N-terminal domain of the archaeal D-family DNA polymerase small subunit reveals evolutionary relationship to eukaryotic B-family polymerases” (2010) FEBS Lett., 584, 3370-3375.

  3. Kuwahara, Y., Unzai, S., Nagata, T., Hiroaki, Y., Yokoyama, H., Matsui, I., Ikegami, T., Fujiyoshi, Y., and Hiroaki, H. “Unusual thermal disassembly of the SPFH domain oligomer from Pyrococcus horikoshii” (2009) Biophys. J., 97, 2034-2043.

  4. Kuwahara, Y., Ohno, A., Yokoyama, H., Matsui, I., Tochio, H., Shirakawa, M., and Hiroaki, H. ”The solution structure of the C-terminal domain of NfeD reveals a novel membrane-anchored OB-fold” (2008) Protein Sci., 17, 1915-1924.

  5. Y. Urushibata, S. Ebisu, and I. Matsui “A thermostable dolichol phosphoryl mannose synthase responsible for glycoconjugate synthesis of the hyperthermophilic archaeon Pyrococcus horikoshii” (2008) Extremophiles, 12, 665-676.

  6. H. Yokoyama, S. Hamamatsu, S. Fujii, and I. Matsui “Novel dimer structure of a membrane-bound protease with a catalytic Ser-Lys dyad and its linkage to stomatin” (2008) J. Synchrotron Radiat. 15, 254-257.

  7. H. Yokoyama, S. Fujii, and I. Matsui “Crystal structure of a core domain of stomatin from Pyrococcus horikoshii Illustrates a novel trimeric and coiled-coil fold” (2008) J. Mol. Biol., 376, 868-878.

  8. N. Ito, I. Matsui, and E. Matsui “Molecular basis for the subunit assembly of the primase from an archaeon Pyrococcus horikoshii” (2007) FEBS J., 274, 1340-1351.

  9. H. Sawai, J. Nagashima, M. Kuwahara, T. Tamura, and I. Matsui “Difference in substrate specificity of C(5)-substituted or C(5)-unsubstituted pyrimidine nucleotides by DNA polymerases from thermophilic bacteria, archaea, and phages”(2007) CHEMISTRY & BIODIVERSITY, 4, 1979-1995.

  10. H. Yokoyama, E. Matsui, T. Akiba, K. Harata, and I. Matsui “Molecular structure of a novel membrane protease specific for a stomatin homolog from the hyperthermophilic archaeon Pyrococcus horikoshii”(2006) J. Mol. Biol., 358, 1152-1164.

  11. G. Perugino, P. Falcicchio, M. M. Corsaro, I. Matsui, M. Parrilli, M. Rossi, and M. Moracci “Preparation of a glycosynthase from the β-glycosidase of the hyperthermophilic Archaeon Pyrococcus horikoshii” (2006) Biocatalysis and Biotransformation, 24, 23-29.

  12. H. Yokoyama and I. Matsui “A novel thermostable membrane protease forming an operon with a stomatin homolog from hyperthermophilic archaeon Pyrococcus horikoshii ” (2005) J. Biol. Chem., 280, 6588-6594

  13. X-F. Tang, Y. Shen, E. Matsui, and I. Matsui “Domain topology of the DNA polymerase D complex from a hyperthermophilic archaeon Pyrococcus horikoshii” (2004) Biochemistry, 43, 11818-11827

  14. T. Akiba, M. Nishio, I. Matsui, and K. Harata “X-ray structure of a membrane-bound β-glycosidase from the hyperthermophilic archaeon Pyrococcus horikoshii” (2004) Proteins, 57, 422-431

  15. E. Matsui, J. Abe, H. Yokoyama, and I. Matsui “Aromatic Residues Located Closely to the Active Center are Essential for the Catalytic Reaction of FEN-1 from Hyperthermophilic Archaeon Pyrococcus horikoshii “ (2004) J. Biol. Chem., 279, 16687-16696

  16. Y. Shen, X-F. Tang, E. Matsui, and I. Matsui “A 21-aa peptide from the cysteine cluster II of the family D DNA polymerase from Pyrococcus horikoshii stimulates its nuclease activity which is Mre11-like and prefers manganese ion as the co-factor“ (2004) Nucleic Acids Res., 32, 158-168

  17. Weng L., Feng Y., Ji X., Cao S., Kosugi Y., and Matsui I. “Recombinant expression and characterization of an extremely hyperthermophilic archaeal histone from Pyrococcus horikoshii OT3” (2004) Protein Expr. Purif.. 33, 145-152


(3)口頭発表と招待講演
  1. I. Matsui “Partial purification of the oligomeric structure of stomatin and STOPP from Pyrococcus horikoshii to comprehend molecular mechanism to form vesicles” JST-CNRS Joint Seminar 2010「Membranes and lipid vesicles from archaea, bacteria, and eukarya」フランス パスツール研究所 (2010/9/22)

  2. I. Matsui “Introduction of the JST-CNRS joint project” JST-CNRS Joint Seminar 2009「Novel virus-like vesicles and their molecular function produced by hyperthermophilic archaea」つくば研究交流センター (2009/10/30)

  3. I. Matsui, E. Matsui, T. Akiba, K. Harata and H. Yokoyama “A novel membrane protease linking to membrane stomatins from the hyperthermophilic archaeon Pyrococcus horikoshii” The abstract of The 6th International Congress on Extremophiles, Extremophiles 2006, p. 68, France、Brest (2006/9/17)

  4. H. Yokoyama and I. Matsui “A novel thermostable membrane protease forming an operon with a stomatin homolog from hyperthermophilic archaeon Pyrococcus horikoshii” The abstract of The Pacifichem 2005 Congress, Extrozyme: Function, Structure and Application, p. 251, USA, Hawaii (2005/12/14)

  5. I. Matsui, XF. Tang, Y. Shen, H. Yokoyama, and E. Matsui “The Molecular Machinery of the Archaeal DNA Replication in Hyperthermophiles - Pyrococcus horikoshii as a Model System” The abstract of International Conference Thermophiles 05, p. 66, Australia, Gold Coast (2005/9/18)

  6. Y. Shen, XF. Tang, E. Matsui, and I. Matsui “Subunit interaction and regulation of activity through the terminal domains of the family D DNA polymerase from Pyrococcus horikoshii” The abstract of International Conference Thermophiles 2003, p. 42, England, Exeter (2003/9/15)

  7. I. Matsui, E. Matsui, Y. Shen, XF. Tang, S. Darnis, J. Abe, M. Nishio, H. Kikuchi, Y. Kawarabayasi, and K. Harata “Novel functions of proteins involving in primer processing during DNA replication of a hyperthermophile Pyrococcus horikoshii” The abstract of The 4th International Congress on Extremophiles, Extremophiles 2002, p. 107, Italy、Naples (2002/9/22)

  8. FW. Cui, H. Ishida, K. Ishikawa, Y. Kosugi, Y. Koyama, E. Matsui, S. Kawasaki, Y. Kawarabayasi, and I. Matsui “Signal recognition protein 54 homologue from Pyrococcus horikoshii can bind to GTP and E. coli 4.5S RNA helix 8” The abstract of International Congress on Extremophiles ’98, p. 60, Japan, Yokohama (1998/1/18)

  9. I. Matsui, K. Ishikawa, E. Matsui, S. Miyairi, S. Fukui, and K. Honda “A Mutant α-Amylase with Enhanced Activity Specific for the Short Substrates” in “Enzymatic Degradation of Insoluble Carbohydrates” 第207回米国化学会年会 農業と食品化学部会要旨集、p. 40, USA, San Diego (1994/3/13)


(4)国内外特許
国内特許
  1. 松井郁夫他“新規耐熱性プロテアーゼ”(査定すみ(H21/11/24))
  2. 松井郁夫他“DNAポリメラーゼ活性と3’-5’エキソヌクレアーゼ活性を有する耐熱性ヘテロダイマー酵素およびその製法”(特許3829174)
  3. 松井郁夫他“プライマーゼ活性を有する耐熱性酵素”(特許 3689736)
  4. 松井郁夫他 “パイロコッカス属に属する超好熱細菌由来の耐熱性Flapエンドヌクレアーゼ”(特許3018163)
  5. 松井郁夫他 “DNAポリメラーゼ活性を有する耐熱性酵素”(特許3015878)


外国特許
  1. 松井郁夫他“フラップエンドヌクレアーゼ変異体”(米国公開番号WO 2006/011253 A1).
  2. I. Matsui et al. “DNA Helicase”  (米国登録番号6939699).
  3. I. Matsui et al. “Thermostable Flap endonuclease derived from hyperthermophile bacterium belonging to the genus Pyrococcus” (米国登録番号6251649).


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