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Toward the development of novel technologies for glycomics.

Hiroaki Tateno Laboratory

Research projects


1. Development of novel technolgies for glycomics

We have been developong novel technologies for glycomics. One of the developed technology is high-density lectin microarray containing 96 lectins. We have also developed glycocomjugate microarray for high-throughput analysis of the glycan-binding specificity of glycan-binding proteins. We are also interested in the creation of novel probes for glycome analysis using evolutionary engineering.

2. Glycome analysis of various cells and vesicles from bacteria to mammals

Using the developed system, we have analyzed the glycome of various cells derived from bacretia to mammals, including ES/iPS cells, messenchymal stem cells, pancreatic cancer cells, lactic acid bacteria, and fungi. Recently, we have analyzed the glycome of extracellular vesicles derived from iPS cells.

3. Development of the quality control system of stem cells

Through comprehensive glycome analysis of iPS/ES cells, we have identified a lectin called rBC2LCN, which binds specificallty to ES/iPS cells, but not to differentiated cells. Using rBC2LCN, we have developed staining reagents of ES/iPS cells. We have also developed a nondestructive method to detect ES/iPS cells using cell culture media. Finally, we have developed a reagent to specifically eliminate ES/iPS cells by fusing rBC2LCN with cytotoxic drugs. We have also found that a2-6Sia is a marker of the differentiation potential of messenchymal stem cells.

4. Development of lectin-drug conjugates (LDC) for cancer therapy

We have also found that rBC2LCN binds to pancreatic cancer stem cells. The fusion protein of rBC2LCN with a cytotoxic drug showed strong anti-cancer effect for pancreatic cancer mouse models. We have now challenging to apply LDC for pancreatic cancer thrapy. We have also trying to identify novel lectins for other cancers.

5. Development of diagnostic system for various diseases

We have also been developong novel diagnostic system for various diseases such as cancers and mental illnesses.


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National Institute of Advanced Industrial Science and Technology (AIST)

国立研究開発法人産業技術総合研究所

Central2-12, 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, Japan

〒305-8568 茨城県つくば市梅園1-1-1中央第2-12

TEL&FAX: 029-861-3125